NovaBlock™ Solution

Highest performance solution created to block non-specific binding of PE-tandem dyes as well as NovaFluor labels.

Application note: NovaBlock^TM requires no change to most flow cytometry staining protocols. Prior to staining, in addition to other blocking solutions, add 5 µL of NovaBlock^TM per 1×10⁶ cells and incubate on ice for 5 minutes.

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NovaBlock™ Solution has been specifically formulated to block the non-specific binding of NovaFluor labels, PE and APC tandems (PE-Dazzle™ 594, PE-Cy5, PE-Cy7, APC-Cy7 and APC-Fire™ 750) observed with macrophages and monocytes.

This non-antibody, non-protein based blocking solution has been shown to act as a high performing blocking solution for flow cytometry in stopping the non-specific binding of NovaFluor labels as well as cyanine-based PE and APC tandems.

Monocytes and background are a thing of the past.

Use interchangeably for PE, APC tandems and NovaFluor™ staining in flow cytometry.
Incorporates seamlessly into your current flow cytometry protocol with no additional steps.
Better resolve populations by combining with NovaFluors to combine the spectrally cleanest fluorescent labels with industry-leading background reduction.
Can be used without NovaFluor™ labels as well as a high performance replacement to your current monocyte blocking solution.

Overlay dot plots displaying human PBMC CD3 and CD14 expression following no block (yellow), NovaBlock^TM (blue), and BioLegend® True-Stain Mononcyte Blocker^TM (red). Data was assessed on an Attune NxT^TM.

Consistently amazing discovery with these Blocking Solution features:

Incorporates into current staining workflow with zero additional hands-on time
Specially optimized blocking solution for flow cytometry applications
Engineered to enable high resolution biology

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Blocking Solutions

Blocking Solution	Formulation	Purpose	Species Compatibility	Notes
NovaBlock^TM	Non-antibody & non-protein	Blocks non-specific binding of NovaFluors^TM, tandem dyes, and PE/Dazzle 594^TM to monocytes/macrophages.	Human & Mouse	Can be used in addition to Fc block. Formulated as 5 µL/test. Does not impact compensation beads.
True-Stain Monocyte Blocker^TM	Non-antibody	Blocks non-specific binding of tandem dyes and PE/Dazzle 594^TMto monocytes/macrophages.	Human & Mouse
Human Fc Block	Human IgG	Blocks non-specific binding of antibodies to Fc receptors expressed on monocytes/macrophages, granulocytes, B cells, and dendritic cells.	Human	Not recommended for staining human IgG.
Mouse Fc Block	Anti-mouse CD16/CD32 antibody /Anti-mouse 2.4G2 antibody	Blocks non-specific binding of antibodies to Fc receptors expressed on monocytes/macrophages, granulocytes, B cells, and dendritic cells.	Mouse
Irrelevant Protein	BSA/FCS/FBS/human serum/mouse serum/rat serum	Blocks non-specific binding of antibodies to Fc receptors expressed on monocytes/macrophages, granulocytes, B cells, and dendritic cells.	Human & mouse
BD Horizon^TM Brilliant Stain Buffer (or BD Horizon^TM Brilliant Stain Buffer Plus)	Unreported proprietary formulation	Reduces dye-dye interactions between different polymer dyes.	Human & Mouse	Formulated as 50 µL/test (or 10 µL/test). May impact fluorescence of compensation beads.
eBioscience^TM Super Bright Staining Buffer	Unreported proprietary formulation	Reduces dye-dye interactions between different polymer dyes.	Human & Mouse	Formulated as 5 µL/test. Not compatible with UltraComp eBeads^TM.

NovaBlock^TM Compatibility

True-Stain Monocyte Blocker^TM	✓
Human TruStain FcX^TM	✓
TruStain FcX^TM	✓
Irrelevant Protein	✓
BD Horizon^TM Brilliant Stain Buffer	✓
eBioscience^TM Super Bright Staining Buffer	✓
IgG Staining	✓
DNA Intercalating Dyes	X
Amine Reactive Dyes	✓
Compensation Beads	✓

Reduce background from Cyanine-based dyes and NovaFluor™ labels

Performance-tested block solutions serve as the best answer for reducing background observed from monocytes and macrophages binding non-specifically to cyanine-based fluorescent dyes.

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Overlay histogram display of lymphocyte (A) and monocyte (B) CD4 expression assessed by NovaYellow^TM 660 anti-human CD4 combined with NovaBlock^TM (cyan), no block (orange), and unstained cells (white) in a single stain flow cytometry experiment performed on healthy thawed human peripheral blood mononuclear cells (PBMCs). Data was acquired on an Attune NxT^TM(Blue/Red/Yellow/Violet).

Cell Phenotyping by Flow Cytometry

Uncover and better resolve cell population heterogeneity by flow cytometry. In combination with our NovaBlock, NovaFluor labels increase the parameters accessible per cell on extant instrumentation, and enable you to discover more.