Flow Cytometry Panel Design tools including a fluorescence spectrum viewer and fluorescent label selection guide. Use this spectral viewer tool and out selection guide to compare fluorescent label emission as an aid to multicolor flow cytometry panel design.
Please use Google Chrome on a Desktop/laptop computer for the best viewing experience, as this view is optimized for 1366×768.
Our Fluorophores for Flow Cytometry selection guide is an interactive guide enabling you to select and choose the best fluorophore for your experiment. As we currently offer NovaFlour fluorescent labels for the blue, yellow/green, and red laser lines, these labels are compared in terms of brightness (separation index) and spread below.
Fluorescent labels for flow cytometry, including spectral spread (calculated as the sum of spectral spread added to all non-primary channels) and separation index (as a measure of brightness, measured using anti-human CD4-SK3 for all fluorescent labels). All measurements were taken on a 5-laser Cytek™ Aurora.
Fluorophore Selection Navigator of blue, yellow/green, and red- excited fluorescent labels used in flow cytometry. How to use: (1) Mouse over to see the label and its values, (2) Double click on a laser line (e.g. 488 nm, blue) to just show fluorophores that are excited by that laser line, (3) you can make a selection by selecting an area of the graph.
Trademarks are the property of their respective owners. Use of them does not imply any affiliation with or endorsement by them.
Alexa Fluor®, eFluor®, LIVE/DEAD™, Qdot™, Super Bright, DyLight, CellTrace, CellTracker, MitoSOX, and MitoTracker are trademarks of Thermo Fisher Scientific. APC/Fire, PE/Dazzle, Spark Blue, Zombie Aqua, Zombie Green, Zombie Red, Zombie Violet, Zombie Yellow, Zombie NIR, and PE/Fire are trademarks trademarks of BioLegend,Inc. Brilliant Violet™ is a trademark of Sirigen Group Ltd. BD Horizon™, Brilliant Blue (BB), Brilliant Violet (BV), Brilliant UV (BUV), and APC-R700 are trademarks of BD Biosciences. CF® is a registered trademark of Biotium. Cy® and CyDye® are registered trademarks of GE Healthcare. DRAQ5 and DRAQ7 are registered trademarks of Biostatus Limited. Ghost Dye, redFluor, and violetFluor are registered trademarks of Tonbo Biosciences. Janelia Fluor is a registered trademark of Tocris Bioscience. KIRAVIA Dyes™ and KIRAVIA Blue 520™ are registered trademarks of SONY Corporation. Krome Orange is a registered trademark of Beckman Coulter. Northern Lights is a registered trademark of R&D Systems. NovaBlue, NovaYellow, NovaRed, NovaFluor, and High Resolution Biology are registered trademarks of Phitonex, Inc. Vio is a registered trademark of Miltenyi Biotec. Allophycocyanin (APC) conjugates: US Patent No. 5,714,386 PE-Cy7: US Patent No. 4,542,104 APC-Cy7: US Patent No. 5,714,386. DsRed and Fruit Fluorescent Proteins (e.g. mCherry): U.S. Patent No. 7,005,511; 7,157,566; 7,393,923 and 7,250,298.