In a Mexican stand-off, neither opponent appears to have a measurable advantage…this is clearly not the case when comparing NovaFluors to Tandem dyes.
First let’s look at the similarities…
Both techniques utilise Förster resonance energy transfer (FRET), which is based upon the transfer of energy from an excited donor fluorophore to a close-by acceptor fluorophore, resulting in enhanced fluorescence emission of the acceptor (Citation).
However, while tandem dyes are composed of two covalently attached fluorescent molecules, and therefore prone to degradation, the phiton structure in the NovaFluors holds both the donor and acceptor dye rigidly in place as described here. What this means is that they are far more stable than tandem dyes.
So NovaFluors tick all the boxes…
- Do not break down leading to false positive events
- Do not break down in fixative solutions as described in an earlier blog
- Spectrally stable at room temperature for at least two weeks
- Consistent from lot-to-lot
- Easy to Compensate
In this Mexican standoff between Tandem dyes and NovaFluors there is only one one clear winner. As Dirty Harry says…”You’ve got to ask yourself a question: ‘do I feel lucky? …”